New therapy offers hope for osteoarthritis sufferers

Steven Ghivizzani

Steven Ghivizzani

Patrick Colahan

 

by Czerne M. Reid

University of Florida researchers are developing a gene therapy technique that could help both humans and horses fight osteoarthritis, a debilitating condition that causes inflammation and deterioration of the joints. The goal is to create a one-time treatment that works long term.

The research team received a highly competitive one-year, $900,000 grant from the National Institute of Health’s National Institute of Arthritis, Musculoskeletal and Skin Disease to fund the work. The new effort will expand laboratory studies into trials that better approximate osteoarthritis in humans.

The work will involve the use of viruses, called adeno-associated viruses, or AAV, as vehicles to deliver genetic material to the joints of horses, where it would produce a therapeutic protein directly at the site of the disease.

“We’re uniquely poised to do this study because UF has a leading program in equine medicine and research and is one of the homes of AAV technology,” said principal investigator Steven Ghivizzani, a professor of orthopaedics and rehabilitation in the UF College of Medicine, and a member of the UF Genetics Institute. Researchers at UF’s Powell Gene Therapy Center are among the pioneers of AAV technology and gene therapy applications for a number of diseases.

Osteoarthritis, the most common type of arthritis, is a chronic condition that affects large weight-bearing joints such as the knees and hips. In osteoarthritis, the cartilage in the joints that usually allows bones to move smoothly over each other wears away, causing bones to rub. The result is pain, stiffness and swelling.

There is no cure for osteoarthritis.

Researchers are testing the treatment

Joint replacement surgery can help ease the disabling effects of the condition. The few medicines that exist for osteoarthritis mostly offer only limited symptom relief. In addition, those drugs can have unwanted consequences. Corticosteroid injections, for example, which are given to both people and horses, also suppress other healthy activities in the joint, such as processes important for healing. The injections also have to be administered repeatedly, which increases the chance of infection.

In contrast, the new gene therapies being developed at UF would require a one-time treatment and would not hinder the body’s healing processes.

The researchers are devising a gene therapy approach that would allow continued production of a therapeutic protein within the joints, directly at the disease site. Unlike existing drugs, the potential one-time treatment would not only address symptoms but also change the course of the disease.

Previous studies in small animals such as rats demonstrated that delivery of the gene therapy resulted in meaningful levels of gene expression within affected joints. The researchers will examine how that translates to the larger joints of horses, which are more similar to human joints in terms of size, tissue structure and weight-bearing stance.

“We hope that this will be at least the first step in a therapy that will benefit both people and animals,” said Patrick Colahan, a board-certified equine surgeon in the UF College of Veterinary Medicine and co-investigator on the study. “It has the potential to help lots of different species, and from a veterinarian’s perspective, that’s what we’d like.”

Steven Ghivizzani, ghivisc@ortho.ufl.edu

Patrick Colahan, colahanp@ufl.edu